Genesis of granulation and scar tissue in otitis media: a temporal bone study

Sixty human temporal bones with evidence of middle ear exudates, from the temporal bone collection of the University of Minnesota, USA are included in this study. Temporal bones has been removed at autopsy, routinely fixed, decalcified dehydrated, embedded in celloidin, and sectioned every 20 microns. Each tenth section had been stained with hematoxylin and eosin and prepared for light microscopy. Results showed that sequential ordering of morphopathologic events, has helped to develop insight regarding the pathogenesis of granulation tissue. Four man varieties of fibrous/granulation tissue, are recognized and described. It was concluded that the pattern of genesis of the fibrocystic tissue is skeletonized. The study presented histologic documentation that the fibrocystic granulation tissue arise in the lumen from altering intraluminal contents

Histomorphometric mapping of melanocytes in the inner ear: a study of human temporal bones

The inner ear in 100 human temporal bones of various age groups was mapped out for ten different locations on each bone, using semiquantitative histomorphometric measures to assess the distribution of pigmentary cells in the labyrinth in the various age groups. The mean pigmentary intensity of the various inner ear location in the various age groups was assessed and tabulated. It was concluded that possible functional correlations to the different pigmentary distributions are suggested. A chronological alteration in pigmentary pattern was shown and was related to the development of the inner ear and to the developmental period during which hearing function is established. Pigment was detected in the subepithelial space of the round window niche and is explained by the migratory ability of the melanocyte